ࡱ>  Mjbjb77 /]]$3"""""""""8"|Q$"B\ & /////2=4@@@@@@@bCF@"5//55@5""//A555!5j"/"/@55@55""5_ʓ"5F55$ A0B5F5F55"5555 O!:    BIOSAFETY CHECKLIST (BSL-2) Building Room: FORMTEXT      Department: FORMTEXT      PI: FORMTEXT      Phone Number: FORMTEXT      Inspector (s): FORMTEXT      Inspection Date: FORMTEXT       FORMTEXT       Biosafety Level 2YesNoN/AComments (additional space on p.2)A. Standard Microbiological Practices1. The Principal Investigator (PI) establishes and enforces policies that limit access to the lab. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      2. Persons must wash hands after working with potentially hazardous materials and before leaving the lab.  FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      3. Eating, drinking, storing food, applying cosmetics, etc., is not permitted in the lab. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      4. Mouth pipetting prohibited; mechanical pipetting devices must be used. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      5. Policies for the safe handling of sharps, such as needles, scalpels, pipettes, and broken glassware must be developed and implemented. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      6. Perform procedures to minimize the creation of splashes and aerosols. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      7. Decontaminate work surfaces after completion of work and after any spill or splash of potentially infectious material with disinfectant. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      8. Decontaminate all infectious material before disposal using an effective method. Durable leak proof containers must be used if decontamination takes place outside of lab. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      9. An effective integrated pest management program is required. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      10. A sign incorporating the universal biohazard symbol must be posted at the entrance to the lab. Should show agent, BSL level, PPE, name, phone nos. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      11. PI must ensure that lab personnel receive appropriate training regarding their duties, precautions to prevent exposures and exposure evaluation procedures. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      B. Special Practices (When required):1. All persons entering the lab must be advised of the hazards and meet specific entry/exit requirements. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      2. Lab personnel must be provided medical surveillance and offered appropriate immunizations for agents handled in the lab. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      3. Laboratory specific biosafety manual/Standard Operating Procedures (SOP) must be prepared and adopted as policy. This manual must be available, accessible and read and understood by all. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      4. The PI must ensure that lab personnel demonstrate proficiency with BSL-2 microbiological practices before working with these agents.  FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      5. Potentially infectious materials must be placed in a durable, leak proof container during collection, handling, processing, storage, or transport. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      6. Lab equipment should be routinely decontaminated, as well as after spills, splashes, or other potential contamination. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      7. Incidents that may result in exposure to infectious materials must be immediately evaluated and treated according to procedures in the biosafety manual/SOPs. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT       a. All such incidents must be reported to the PI. PI must also report incident to the RIC IBC. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT       b. Medical evaluation, surveillance, and treatment should be provided when required and appropriate records maintained. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      8. Animals and plants not associated with the work being performed are not to be permitted in the lab. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      9. All aerosol generating procedures are conducted in a BSC or other appropriate physical containment devices (not laminar flow workbenches) such as sealed centrifuge rotor cups/heads. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT       C. Safety Equipment (Primary Barriers)YesNoN/AComments (additional space on p.2)1. Biosafety cabinet (Class II), certified annually, and other containment devices or PPE used when:  a. Potential for aerosols or splashes exists. These may include centrifuging, grinding, blending, inoculating animals intranasally, harvesting infected tissues, etc. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT       b. High concentrations/titers or large volumes of agents are used. These may be centrifuged outside the BSC using sealed rotor cups/heads. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      2. Face protection used for work outside BSC that may generate splashes. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      3. Lab coats worn and removed prior to leaving lab. Lab coats must not be taken home. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      4. Gloves must be worn to protect hands from exposure to agents. Glove selection should be based on risk assessment. Gloves must not be reused and must be removed prior to leaving lab. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      D. Laboratory Facilities (Secondary Barriers) FORMTEXT      1. Lab doors should be self closing and have locks in accordance with RIC policies. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      2. Labs must have a hand washing sink. It should be located near the exit door. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      3. Lab should be designed so that it can be easily cleaned and decontaminated. Carpets and rugs not permitted. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      4. Benchtops impervious to water and resistant to chemicals FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      5. Lab furniture (chairs, tables, etc.) is appropriate for loading and use. Spaces accessible for cleaning FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      6. BSCs must be installed properly to avoid room air fluctuations that might impede proper functioning of the cabinet. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      7. Eyewash readily available and tested periodically. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      8. Lab windows that open to exterior are not recommended. If a lab does have such windows, they must be fitted with screens FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      9. Negative room airflow relative to the hallway recommended. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      10. Vacuum lines should be protected with HEPA (High Efficiency Particulate Air) filters. Liquid disinfectant traps may be required. FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      11. HEPA filtered exhaust air from a Class II BSC can be safely recirculated if cabinet is tested and certified annually.  FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX BSC Certification Date:  FORMTEXT      12. Are autoclaving procedures verified? If yes, explain how: FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT       Training of PersonnelYesNoN/ACommentsDocumented lab safety training? FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      Documented bloodborne pathogens training (if required)? FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT      Documented chemical safety training (if required)? FORMCHECKBOX  FORMCHECKBOX  FORMCHECKBOX  FORMTEXT       ADDITIONAL COMMENTS:  FORMTEXT       The Principal Investigator is responsible for full compliance with the policies, practices and procedures set forth in the Biosafety in Microbiological and Biomedical Laboratories  5th Edition, the Rhode Island College Biosafety Handbook and the laboratorys specific standard operating procedures. The PI is responsible for assuring the appropriate training of employees and for correcting errors and unsafe working conditions. 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